Carbonic Anhydrases are Influenced by the Size and Symbiont Identity of the Aggregating Sea Anemone Anthopleura elegantissima
June 08, 2020 | Biology
Reading time: 1 minute
Get your Biology news fix anytime you want it thanks to Lab.Equipment's freshly acquired articles from trusted online sources. Read this one first appearing on Journal of Experimental Biology (JEB):
Jack Cushman Koch, E. Alan Verde, and Virginia M. Weis
Carbonic anhydrases (CA; EC 184.108.40.206.) play a vital role in dissolved inorganic carbon (DIC) transport to photosynthetic microalgae residing in symbiotic cnidarians. The temperate sea anemone Anthopleura elegantissima can occur in three symbiotic states: hosting Breviolum muscatinei (brown), Elliptochloris marina (green) or without algal symbionts (aposymbiotic). This provides a basis for A. elegantissima to be a model for detailed studies of the role of CA in DIC transport. This study investigated the effects of symbiosis, body size, and light on CA activity and expression, and suggests that A. elegantissima has a heterotrophy-dominated trophic strategy. We identified putative A. elegantissima CA genes and performed phylogenetic analyses to infer subcellular localization in anemones. We performed experiments on field-collected anemones to compare (1) CA activity and expression from anemones in different symbiotic states, (2) CA activity in brown anemones as a function of size, and (3) CA activity in anemones of different symbiotic states that were exposed to different light intensities. Carbonic anhydrase activity in brown anemones was highest, whereas activity in green and aposymbiotic anemones was low. Several CAs had expression patterns that mirrored activity while another had expression that was inversely correlated to activity suggesting that symbionts may induce different DIC transport pathways. Finally, CA activity was inversely correlated with anemone size. Our results suggest that the observed CA activity and expression patterns are not only affected by symbiosis but also by other factors in the host physiology including trophic strategy as it relat... Want to read more? Visit the Journal of Experimental Biology (JEB) Blog.
If you're in the Bio industry and are in need of multipurpose Bio lab equipment for your basic or advanced applications, then you are in the right place. Lab.Equipment presents an extensive collection of equipment best for Bio laboratories. Check out our recommended Biology products available and ready for delivery such as:
We showcase multifunction and user-friendly products from well-trusted manufacturers including:
You can also rely on Lab.Equipment for a variety of reliable equipment best-fit for applications such as microbiology, environmental, clinical, and more. Boost your laboratory efficiency and productivity by shopping accurate laboratory equipment here.