NKX3-1 Is a Useful Immunohistochemical Marker of EWSR1-NFATC2 Sarcoma and Mesenchymal Chondrosarcoma

Be in the know about the latest in the Pathology industry. Lab.Equipment sees to it that you get your Histopathology news on a day-to-day basis and only from reliable online sources. Today’s article was first published on The American Journal of Surgical Pathology:

NK3 homeobox 1 (NKX3-1) is widely accepted as a highly sensitive and specific marker for prostatic adenocarcinoma. Prompted by published transcriptome data showing upregulation of NKX3-1 mRNA expression in EWSR1-NFATC2 sarcoma, we explored the utility of NKX3-1 immunohistochemistry in sarcoma diagnosis. We applied NKX3-1 immunohistochemistry to 11 EWSR1-NFATC2 sarcomas and 168 mimics using whole tissue sections. All EWSR1-NFATC2 sarcomas consisted of uniform small round or ovoid cells, all except 1 showing at least focally the typical growth pattern of nests, cords, or trabeculae within a fibrous/myxoid background. A variable eosinophilic infiltrate was common. NKX3-1 was expressed in 9 of 11 (82%) EWSR1-NFATC2 sarcomas, often diffuse and of moderate or strong intensity. All 12 mesenchymal chondrosarcomas tested were also positive for NKX3-1, with over half showing diffuse staining and moderate or strong intensity. The positive staining was seen only in the primitive small round cell component, whereas the cartilaginous component was mostly negative. Although 1 of 30 osteosarcomas showed focal NKX3-1 positivity, all the remaining 155 cases tested, including 20 Ewing sarcomas, 20 myoepithelial tumors, 11 ossifying fibromyxoid tumors, and 1 FUS-NFATC2 sarcoma were negative for NKX3-1. Our study provides the first evidence that EWSR1-NFATC2 sarcoma and Ewing sarcoma could be distinguished immunohistochemically, adding to the accumulating data that these tumors are phenotypically distinct. We suggest that NKX3-1 may ha... Want to read more? You can view the full article in the AJSP Blog.

If you're in the Pathology industry and are in need of top quality Pathology lab equipment for tissue handling or tissue preparation or basic and advanced applications, then you are in the right place. Lab.Equipment distributes a large range of products for workrooms analyzing and running different histopath tests. Go through our highly-recommended Pathology equipment including:

• Mixers
• Lab Centrifuges
• Lab Pipettes
• Laboratory Stirrers
• Laboratory Balances

We supply multiuse products designed and produced by trusted MFGs such as:

• Troemner
• AnD Weighing
• Aczet
• Radwag
• Accuris

Focusing on other applications? You can depend on Lab.Equipment for much needed assistance. Raise workroom productivity with lab equipment for other industries including microbiology, forensics, environmental, and more. Check out what you need for your application here.