Stable Mitochondrial CICIII2 Supercomplex Interactions in Reptiles Compared to Homeothermic Vertebrates
June 05, 2020 | Biology
Reading time: 1 minute
Sourced by Lab.Equipment from the Journal of Experimental Biology (JEB), here is this week's Biology industry article for you:
Amanda Bundgaard, Andrew M. James, Michael E. Harbour, Michael P. Murphy, and Angela Fago
The association of complex I (CI), complex III (CIII) and complex IV (CIV) of the mitochondrial electron transport chain into stable high-molecular weight supercomplexes (SCs) has been observed in several prokaryotes and eukaryotes, but among vertebrates it has only been examined in mammals. The biological role of these SCs is unclear but suggestions so far include enhanced electron transfer between complexes, decreased production of the reactive oxygen species (ROS) O2·– and H2O2, or enhanced structural stability. Here, we provide the first overview on the stability, composition and activity of mitochondrial SCs in representative species of several vertebrate classes to determine patterns of SC variation across endotherms and ectotherms. We found that the stability of the CICIII2 SC and the inclusion of CIV within SC varied considerably. Specifically, when solubilized by the detergent DDM, mitochondrial CICIII2 SCs were unstable in endotherms (birds and mammals) and highly stable in reptiles. Using mass-spectrometric complexomics, we confirmed that the CICIII2 is the major SC in the turtle, and that 90% of CI is found in this highly stable SC. Interestingly, the presence of stable SCs did not prevent mitochondrial H2O2 production and was not associated with elevated respiration rates of mitochondria isolated from the examined species. Together, these data show that SC stability varies among vertebrates and is greatest in poikilothermic reptiles and weakest in endotherms. This pattern suggests an adaptive role of SCs to varying body temperature, but not necessarily a... Read more of this in the Journal of Experimental Biology (JEB) Blog.
Handling Biology applications like cultivation or agriculture and looking for top quality Bio equipment for your facility? You can trust Lab.Equipment for a portfolio of Biology products with a plethora of accessories available designed and manufactured by major brands such as:
We deliver robust and cost-efficient products highly recommended for any Biology application like: