Visualization of Two Architectures in Class-II CAP-dependent Transcription Activation

April 27, 2020 | Biology

Enjoy your Biology industry news fix anytime you want it. Lab.Equipment presents you this article freshly curated from PLOS Biology:

by Wei Shi, Yanan Jiang, Yibin Deng, Zigang Dong, Bin Liu

Transcription activation by cyclic AMP (cAMP) receptor protein (CAP) is the classic paradigm of transcription regulation in bacteria. CAP was suggested to activate transcription on class-II promoters via a recruitment and isomerization mechanism. However, whether and how it modifies RNA polymerase (RNAP) to initiate transcription remains unclear. Here, we report cryo–electron microscopy (cryo-EM) structures of an intact Escherichia coli class-II CAP-dependent transcription activation complex (CAP-TAC) with and without de novo RNA transcript. The structures reveal two distinct architectures of TAC and raise the possibility that CAP binding may induce substantial conformational changes in all the subunits of RNAP and transiently widen the main cleft of RNAP to facilitate DNA promoter entering and formation of the initiation open complex. These structural changes vanish during further RNA transcript synthesis. The observations in this study may reveal a possible on-pathway intermediate and suggest a possibility that CAP activates transcription by inducing intermediate state, in addition to the previously proposed stabilization mechanism...Want to read more? Check the full article in the PLOS Biology Blog

In the Bio industry and are scouting for multipurpose Bio lab equipment to complete your laboratory? Check out Lab.Equipment’s collection of Biology products built by globally trusted brands such as:

Find valuable and user-friendly Biology equipment available on Lab.Equipment like:

Fulfilling clinical, forensics, cannabis applications? Rely on Lab.Equipment to boost your efficiency and productivity. Get precise lab equipment for whatever application here.

Back To Top
We are fully compliant with the GDPR laws. We promise to safeguard your data and protect your privacy rights.