Molecular Heterogeneity of Endometrioid Ovarian Carcinoma: An Analysis of 166 Cases Using the Endometrial Cancer Subrogate Molecular Classification
June 16, 2020 | Histopathology
Reading time: 1 minutes
Be in the know about the latest in the Pathology industry. Lab.Equipment sees to it that you get your Histopathology news on a regular basis and only from credible sources. Here is today's article first published on The American Journal of Surgical Pathology:
Endometrioid ovarian carcinoma (EOC) has clinical and biological differences compared with other histologic types of ovarian carcinomas, but it shares morphologic and molecular features with endometrioid endometrial carcinoma. To analyze the molecular heterogeneity of EOC according to the new molecular classification of endometrial cancer and to evaluate the prognostic significance of this molecular classification, we have analyzed 166 early-stage EOC by immunohistochemistry for mismatch repair proteins and p53 expression, and by Sanger sequencing for the exonuclease domain of polymerase epsilon (POLE EDM). In addition, we have carried out next-generation sequencing analysis of tumors with POLE EDM mutations to confirm the ultramutated profile. Eight tumors carried POLE EDM mutations and were classified as ultramutated (5%), 29 showed mismatch repair deficiency and were classified as hypermutated (18%), 16 tumors had a mutated pattern of p53 expression and were classified as p53 abnormal (11%), and 114 tumors did not have any of the previous alterations and were classified as no specific type (66%). Five tumors showed >1 classification criteria. The frequencies of ultramutated and hypermutated tumors were lower in EOC compared with the frequency reported in endometrial cancer. Subrogate molecular groups differed in both morphologic features (histologic grade, squamous and morular metaplasia, and necrosis) and immunohistochemical expression of several biomarkers (ARID1A, nuclear β-catenin, estrogen receptors, Napsin A... Want to read more? Visit the AJSP Blog.
Managing Pathology applications like specimen handling or tissue staining and looking for quality Surgical Pathology lab equipment? Trust Lab.Equipment for a comprehensive selection of lab products manufactured by recognized brands including:
Find robust and valuable Pathology lab equipment recommended for basic and advanced Pathology applications by browsing our product portfolio of:
Running other applications? You can count on Lab.Equipment for needed assistance. Improve laboratory productivity with lab equipment for other industries including pharmaceutical, environmental, forensics, and more. Find what you need for your application here.